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. 2012 Feb 23;8(2):e1002554. doi: 10.1371/journal.ppat.1002554

Figure 6. Phosphorylation of CDC20 in schizonts, activated gametocytes and ookinetes.

Figure 6

Schizonts (Sch), activated gametocytes (AG) and ookinetes (Ook) purified using the corresponding Nycodenz protocols were metabolically labelled with 32P-orthophosphate for 30 min, lysed, and GFP-tagged CDC20 was immunoprecipitated using GFP-TRAP beads. A. Phosphorylation of CDC20-GFP in schizonts, activated gametocytes and ookinetes as assessed by autoradiography. B. Protein expression levels by Western Blot using a polyclonal anti-GFP antibody.