Figure 6. Electron microscopy on negatively stained G_th at pH 7.5, pH 6.7 and pH 5.7.

(A) At pH 7.5, no oligomer of G_th is detected and a definite molecular shape cannot be identified. (B) At pH 6.7 a few G_th assemblies can be detected. (C) At pH 5.7 G_th trimers are observed assembled either in lattice via lateral interactions or in rosette-like assemblies. Protein concentration was 0.1 mg/mL for all 3 samples. The scale bar is for 100 nm. (D) Close up view of G_th rosettes observed at pH 6.7 and 5.7. Notice the differences in the aspect of the protein assemblies. The scale bar is for 20 nm.