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. 2012 Feb 23;7(2):e31846. doi: 10.1371/journal.pone.0031846

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Endo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot for some substrates of DDB1-Cul4A ubiquitin ligase using lysates of hepatocytes isolated from DDB1^F/F and DDB1^F/F;Alb-Cre^+/+ mice. (B) Western blot for DDB1 and p21 using lysates of cytoplasmic fraction (C) and nuclear fraction (N) prepared from MEFs. (C) IHC staining for DDB1 and Ki67 on liver sections from DDB1^F/F;Alb-Cre^+/−, DDB1^F/F;p21^−/− and DDB1^F/F;Alb-Cre^+/−;p21^−/− mice. (D) Co-IF staining for A6 and DDB1 on DDB1^F/F;p21^−/− and DDB1^F/F;Alb-Cre^+/−;p21^−/− liver sections. Arrows in DDB1^F/F;Alb-Cre^+/−;p21^−/− mice indicate A6 positive oval cells.