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. 2012 Feb 23;7(2):e32448. doi: 10.1371/journal.pone.0032448

Figure 3. Effect of ET-1 on the expression of HIF-1α in Cx43-silenced astrocytes.

Figure 3

Astrocytes were transfected with NT-siRNA or with Cx43-siRNA as indicated. After 48 h, cells were incubated in the absence (control, C) or presence of 0.1 µM ET-1 for 24 h. HIF-1α levels were analysed by Western blot. Note that the differences between ET-1 and Control in Cx43-silenced astrocytes were lower than those found between ET-1 and Control in NT-siRNA or in non-transfected astrocytes. The results are expressed as percentages of the level found in control non-transfected cells. ***p<0.001 versus the corresponding controls; #p<0.05 versus the corresponding non-transfected cells. n.s. not significant.