. 2012 Feb 23;7(2):e32343. doi: 10.1371/journal.pone.0032343

Table 3. Leu-Enkephalin and Insulin B chain dual substrate assays.

Mutant NEP	leu-ENK	Insulin B Chain	Ratio leu-ENK insulin B chain
	pmole/min/ng	pmole/min/ng
NEP	1.16±0.06 (0.61)	0.20±0.04 (0.23)	5.8
NEP^F563V	0.23±0.03 (0.74)	0.04±0.01 (0.67)	5.8
NEP^F563L	0.97±0.06 (0.62)	0.19±0.03 (0.20)	5.1
NEP^F563M	0.69±0.11 (0.57)	0.11±0.02 (0.52)	6.3
NEP^F563I	0.62±0.03 (0.58)	0.02±0.01 (0.15)	31.0
NEP^S546E	0.76±0.14 (0.68)	0.25±0.01 (0.43)	3.0

The numbers in parenthesis indicate activity relative to the uninhibited values.

Reactions were carried out at 37°C at in 20 mM MES buffer, pH 6.5 containing 15 µM insulin B chain and 64 µM leu-ENK. Activity was measured and statistical analysis carried out as in Table 2 .