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. 1991 Aug 11;19(15):4247–4251. doi: 10.1093/nar/19.15.4247

Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene.

Z A Shabarova 1, I N Merenkova 1, T S Oretskaya 1, N I Sokolova 1, E A Skripkin 1, E V Alexeyeva 1, A G Balakin 1, A A Bogdanov 1
PMCID: PMC328569  PMID: 1870979

Abstract

An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribo nucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degrees C in buffer. The resulting mini-gene was cloned and expressed in vivo and in vitro. We have also found that the polymerase inhibition technique (toe-printing) is a good way to ascertain that translation initiation complexes form in the case of single-stranded DNAs as well. Thus, along with the fully chemical assembly of synthetic genes, a rapid and sufficiently reliable method for determining their ribosome-binding properties was developed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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