Fig. 4.

HPLC carotenoid profiling of control lines, line 1804 carrying the P192L substitution and line 5381 carrying the W180* mutation in Psy1. Carotenoids were measured from fruit at three developmental and ripening stages: Mature Green, Breaker + 3 and Breaker + 7 stages. A Fruit phytoene content expressed as area under the phytoene curve. B Fruit lycopene content expressed in μg of lycopene/mg of fruit fresh weight. C Fruit β-carotene content expressed in μg of lycopene/mg of fruit fresh weight. D Fruit lutein content expressed in μg of lycopene per mg of fruit fresh weight. Significant difference (Student’s t test) in compound content in mutant lines compared to controls are represented with one or more asterisks with the following levels of significance: *P < 0.05; **P < 0.01; ***P < 0.001