Fig. 1.

Genomic organisation of Mal d 1 gene cluster on LG16. a Genetic map of Durello di Forlì LG16. SSRs developed based on the sequences of the two BACs are indicated in bold. b Physical map of the two BAC clones from cv Florina, MC-12 and MC-20. c Physical map of Mal d 1 cluster on LG16 from GD draft genome sequence. In (b) and (c), the Mal d 1 gene positions are indicated as black bars, the pseudogene positions as striped bars and the other genes in the cluster as dotted bars. The isoallergen genes previously known but located for the first time or designated by a new name are underlined, and the new isoallergen genes are indicated in boxes. The arrows indicate gene orientation; ° and ″ are identical sequences; *Mal d 1.12 sequence, but with a gap of 45 bp