Fig. 3.

Alignment of predicted amino acid sequences of Mal d 1 isoforms. Mal d 1 sequences were retrieved from the BAC clones sequences and from Gbrowser of the apple genome sequence. Two Bet v 1 isoforms, Bet v 1.01 and Bet v 1.04, are also included. The sequences are indicated with the isoform name followed by the ID number and the LG in which they are located. The P-loop region is indicated by the dashed box; substitutions between Bet v 1 sequences are indicated as small boxes; the position 45 is highlighted in red and the other amino acids putatively important for IgE recognition are within brackets or large boxes. Important amino acid substitutions are shown as circles