Table 2.
Description of the Mal d 1-like sequences in the MC-12 and MC-20 sequences (FN823234 and FN823235, respectively) analysed with BLASTN software
| Mal d 1 genes features | BLASTN results | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ORF | Mal d 1 gene/allele | Dira | Full length (nt) | CDS (nt) | Intron/Exon | Most similar sequence ID | Descriptionb | SNPs in CDS | SNPs in intron | E value |
| On BAC clone MC-12 | ||||||||||
| ORF6 | Mal d 1.06A02 | ↑ | 602 | 480 | Exon I:184; Intron:122; Exon II: 296 | AY827697 | Malus x domestica clone 220903B10 Mal d 1.06A02 (Mal d 1.06A) gene | 1 | 0 | 0.0 |
| ORF10 | Mal d 1.1001 | ↓ | 961 | 486 | Exon I:184; Intron:475; Exon II: 302 | AY428588 | Malus x domestica clone 1 m Mal d 1-like mRNA | 0 | Not available | 1.00E–156 |
| ORF13 | Mal d 1.0201 | ↓ | 651 | 480 | Exon I:184; Intron:171; Exon II: 296 | AY827654 | Malus x domestica clone 250803B10 Mal d 1.0201 (Mal d 1.02) gene | 1 | 0 | 0.0 |
| ORF17 | Mal d 1.1201 | ↓ | 861 | 486 | Exon I:184; Intron:375; Exon II: 302 | AY822733 | Malus x domestica clone 231103G3 Mal d 1.03G01 (Mal d 1.03G) gene | – | – | 3.00E–25 |
| ORF20 | Mal d 1.11A01 | ↓ | 702 | 495 | Exon I:184; Intron:208; Exon II: 310 | AY428589 | Malus x domestica clone 1n Mal d 1-like mRNA | 0 | Not available | 4.00E–160 |
| ORF23 | Mal d 1.06B02 | ↓ | 633 | 480 | Exon I:184; Intron:153; Exon II:296 | AY827712 | Malus x domestica clone 220903F10 Mal d 1.06B02 (Mal d 1.06B) gene | 0 | 0 | 0.0 |
| ORF25 | Mal d 1ps301 | ↓ | 283 | – | With stop codons and truncated | AY827730 | Malus x domestica clone 220903A7 Mal d 1ps2 pseudogene | – | – | 2.00E–113 |
| ORF28 | Mal d 1.06C02 | ↓ | 608 | 480 | Exon I:184; Intron:128; Exon II:296 | AY827725 | Malus x domestica clone 231103F11 Mal d 1.06C02 (Mal d 1.06C) gene | 1 | 0 | 0.0 |
| ORF29 | Mal d 1ps204 | ↓ | 470 | – | With stop codons | AY827730 | Malus x domestica clone 220903A7 Mal d 1ps2 pseudogene | – | – | 0.0 |
| On BAC clone MC-20 | ||||||||||
| ORF45 | Mal d 1.03G02 | ↑ | 480 | 480 | Intronless | AY822733 | Malus x domestica clone 231103G3 Mal d 1.03G01 (Mal d 1.03G) gene | 5 | – | 0.0 |
| ORF46 | Mal d 1.0703 | ↓ | 480 | 480 | Intronless | AY822717 | Malus x domestica clone 231103B3 Mal d 1.0701 (Mal d 1.07) gene | 5 | – | 0.0 |
| ORF47 | Mal d 1.0903 | ↓ | 480 | 480 | Intronless | AY822721 | Malus x domestica clone 231103C1 Mal d 1.0903 (Mal d 1.09) gene | 0 | – | 0.0 |
| ORF49 | Mal d 1.0801 | ↓ | 480 | 480 | Intronless | AY822719 | Malus x domestica clone 231103F3 Mal d 1.0801 (Mal d 1.08) gene | 0 | – | 0.0 |