Figure 4.

In vitro blast injury of OHSC. OHSC were exposed to 530 ± 17.7 kPa peak incident overpressure, 1.026 ms duration ± 0.017 ms, and 190 ± 10.7 kPa-ms impulse in-air. (A) Injured OHSC experienced increased cell death 4 days following blast. Cell death increased significantly in all regions of injured OHSC as compared to sham-exposed OHSC (*p < 0.05; SEM; Sham n = 21; Injured n = 13). (B) Bright-field and fluorescent images of injured tissue revealed significant PI staining 4 days following injury. (C) Sham-exposed cultures maintained normal morphology with minimal cell death over the 4-day period following injury.