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. Author manuscript; available in PMC: 2012 Feb 24.
Published in final edited form as: Mech Ageing Dev. 2009 Aug 11;130(9):637–647. doi: 10.1016/j.mad.2009.07.007

Figure 3. Validation of array data by real-time quantitative RT-PCR.

Figure 3

cDNAs were prepared from RNA isolated from liver tissue of young (4–6 months) and old (24–28 months) mice injected with 2NP and their non-injected counterparts as described in Materials and Methods. A. Validation of genes differentially expressed in response to 2NP in young and in response to 2NP in old animals. Differential expression as determined by array represents average fold change from 4 arrays for each group (p<0.005). Real-time RT-PCR data represents the average fold change for 10 animals from each group (±SEM, p<0.05). Data is presented as fold change array/fold change real-time. B. Experimental validation of statistically derived differences comparing response in old to response in young (ΔOld:ΔYoung). Differential expression in response to treatment in old relative to young (ΔOld:ΔYoung) was determined by ANOVA (p<0.005). Real-time RT-PCR data represents the average fold change for 10 animals from each group (±SEM, p<0.05). Data is presented as fold change ANOVA/fold change real-time.