FIGURE 5.

Novel miRNAs are associated to RISCs. (A) Newly identified miRNAs were measured in Myc-Ago2–containing RISC immunocomplexes, and their levels are expressed as average fold change compared with control (n = 3). Red and green colors indicate up- and down-regulation, respectively, and modulations are indicated using a log2 scale (−ΔΔCt). (B) Table showing the same values displayed in A in a linear scale. Candidates highlighted in the red square display a statistically significant, greater than 20-fold enrichment. (C) Secondary structures, predicted using RNAfold software (Vienna RNA package), of three representative novel miRNA stem–loops and of one annotated miRNA (miR-210). Mature miRNAs are highlighted in red.