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Hammerhead ribozyme sequence, proposed structure, and site of cleavage (arrow). Shaded regions represent areas of base pairing. (A) Structure resulting in leaderless cI-lacZ mRNA with a 5′-OH after cleavage. (B) Structure resulting in lac-leadered cI-lacZ mRNA with a 5′-OH after cleavage. Sequence in bold identifies the beginning of the cI coding sequence (A) or the lac-untranslated leader sequence (B).
