FIGURE 3.

Ferritin mRNA associates with the heavy polysomes following treatment with hemin, under conditions of global translational arrest. Cytoplasmic lysates from HT1080-GyrB-PKR cells were subjected to polysome profiling with continuous monitoring of A₂₆₀. The cells were previously either left untreated (A) or treated with 100 ng/mL coumermycin (B), 100 μM hemin (C), or both coumermycin and hemin (D). H-ferritin and β-actin mRNA were analyzed by quantitative real-time PCR. The percentage of H-ferritin (E) and β-actin (F) mRNA in the fractions lighter or heavier than 80S is shown on the graphs corresponding to each treatment.