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. 2012 Feb 24;2:286. doi: 10.1038/srep00286

Figure 3. OsNRAMP5 contributes to Mn, Fe and Cd transport.

Figure 3

(a–d) Green fluorescent protein (GFP) fluorescence in onion epidermal cells observed by confocal laser scanning microscopy (a), OsNRAMP5-sGFP fluorescence (b), fluorescence of FM4-64 and (c) overlay of FM4-64 and OsNRAMP5-sGFP. (d) Fluorescence of sGFP only. Scale bar = 50 μm. (e–g) Serial dilutions of yeast cells for Δsmf (Mn uptake mutant) (e), Δfet3fet4 (Fe uptake mutant) (f), Δycf1 (Cd-sensitive mutant) (g), transformed with empty vector (V.C.), OsNRAMP1 or OsNRAMP5.