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. 2012 Feb 24;2:286. doi: 10.1038/srep00286

Figure 4. Characterization of OsNRAMP5i and OsNRAMP5 OX lines.

Figure 4

(a, b) OsNRAMP5 transcription in roots (a) and shoots (b) of wild type, OsNRAMP5i (T5i-1, T5i-2), and OsNRAMP5OX (OX1, OX2) plants (cultivar Tsukinohikari) grown in control hydroponic culture. (c, d) Phenotypes of wild type and T5i plants grown under control (c) and Mn-deficient conditions (d). Scale bars = 10 cm. Root length (e), shoot length (f), SPAD value (g), root (h) and shoot (i) Mn concentrations, root (j) and shoot (k) Fe concentrations of plant grown under control conditions or Mn-deficient (-Mn) conditions. Error bars represent the SD. Columns bars followed by different letters are statistically different according to analysis of variance followed by SNK test (a, p = 0.0013; b, p = 0.0003; e, p = 0.0017; f, p = 0.0009 (Ctrl.) & p = 0.0006 (-Mn); g, p = 0.0001; h, p = 0.0000 (Ctrl.) & p = 0.0067 (-Mn); i, p = 0.0000 (Ctrl.) & p = 0.0004 (-Mn); j, p = 0.0095; k, p = 0.0000; n = 3.