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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Med Sci Sports Exerc. 2012 Jan;44(1):27–38. doi: 10.1249/MSS.0b013e318228879b

FIGURE 3.

FIGURE 3

Immunoblot analysis of expression of markers related to nitric oxide (NO) production/ bioavailability in coronary arterioles from normal fat diet sedentary (NFSed) and exercise (NFEx); high fat diet sedentary (HFSed) and exercise (HFEx). Representative blots are presented at the top of each set of mean values presented as bar graphs. The band sequence on the sample blots, from left to right, for eNOS, ph-eNOS, AKT, and Catalase is: NFSed, NFEx, HFSed, HFEx, NFSed, NFEx, HFSed, HFEx, Std, and Std. For SOD-2, SOD-1, Caveolin, and nitrotyrosine the sequence is: 3 samples of NFSed, 3 samples of NFEx, 3 samples of HFSed, and 3 samples of HFEx. The sequence of the immunoblots, from top to bottom, is as follow; endothelial nitric oxide synthase (eNOS); phospho-eNOS content expressed relative to total eNOS; AKT; catalase; SOD-2; SOD-1; Caveolin; and Nitrotyrosine. Values are means ± SE. Data are normalized so that NFSed values were set to 1.0. * = HFEx greater than HFSed p value < 0.05. # = HF diet values different from NF values.