Skip to main content
. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Cell Stem Cell. 2011 May 6;8(5):566–579. doi: 10.1016/j.stem.2011.03.010

Fig. 2. ANPs are born from QNPs.

Fig. 2

(A,B) In Gli1-CreER/RCE animals, GFP is expressed exclusively in QNPs 12-18 hr after tamoxifen induction. Later (48 hr after the induction in A), asymmetrically dividing QNPs giving rise to ANPs can be observed; lower panel shows a focal plane from the orthogonal projection of the same pair of cells. Plane of division (dotted line) is often parallel to the SGZ. Furthermore (120 hr after the induction in B), separate ANPs can be identified.

(C, D) A pair of QNP and ANP cells in late telophase in the DG of Gli1-CreER/RCE mouse 24 hrs after tamoxifen induction. In D, the midbody is visualized by antibody to Aurora B (arrow; also shown at higher magnification in the inset) to show that such pairs indeed correspond to a newly divided QNP and its daughter cell.

(E) A pair of a QNP and an ANP cell in late telophase in the DG of Nestin-CreER/Z/EG mouse 24 hr after tamoxifen induction.

(F-I) Pairs of QNP and ANP cells (F, G) and of ANP cells (H, I) in telophase in the DG of Nestin-GFP mice; F and I are z-stack maximum projections (5 and 7μm thick, respectively), G and H are single focal planes (1μm thick). Nuclei of dividing cells are visualized with antibodies to BrdU (24 hr after a single pulse) (F, H) or to phosphorylated histone H3 (G, I). The plane of division (dotted line) is most often parallel to the SGZ for the QNP/ANP pairs but can be different for the ANP pairs. Channels for multiple labels are indicated on the figures. Scale bar is 10μm in A-E and 5μm in F-I.

See also Figures S3 and S4.