Table 1.
RT-PCR primers for FOXO1 and inflammation-related genes
| Gene | Cycle | Tm (°C) | Product (bp) | Primers (5′ to 3′) |
|---|---|---|---|---|
| β-Actin | 28 | 60 | 308 | Forward: CATCTCTTGCTCGAAGTCCA |
| Reverse: ATCATGTTTGAGACCTTCAACA | ||||
| FOXO1 | 35 | 62 | 188 | Forward: GCTGCATCCATGGACAACAACA |
| Reverse: CGAGGGCGAAATGTACTCCAGTT | ||||
| CCL20 | 38 | 58 | 342 | Forward: GCAAGCAACTTTGACTGCTG |
| Reverse: CAAGTCCAGTGAGGCACAAA | ||||
| IL-1α | 38 | 65 | 234 | Forward: GTAAGCTATGGCCCACTCCA |
| Reverse: AGGTGCTGACCTAGGCTTGA | ||||
| IL-1β | 38 | 57 | 184 | Forward: CTGAAAGCTCTCCACCTC |
| Reverse: GATCTACACTCTCCAGCTG | ||||
| IL-1R | 38 | 58 | 271 | Forward: TGTGGAAAATCCTGCAAACA |
| Reverse: GGAATCCCTGTACCAAAGCA | ||||
| IL-2 | 38 | 58 | 207 | Forward: GCAACTCCTGTCTTGCATTG |
| Reverse: GCCTTCTTGGGCATGTAAAA | ||||
| IKKα | 38 | 58 | 181 | Forward: GAAGGTGCAGTAACCCCTCA |
| Reverse: ATTGCCCTGTTCCTCATTTG | ||||
| IKKβ | 38 | 60 | 232 | Forward: AACCAGCATCCAGATTGACC |
| Reverse: CTCTAGGTCGTCCAGCGTTC | ||||
| GAPDH | 60 | 77 | Forward: TGTGTCCGTCGTGGATCTGA | |
| Reverse: CCTGCTTCACCACCTTCTTGAT | ||||
| F4/80 | 60 | 223 | Forward: TTTCCTCGCCTGCTTCTTC | |
| Reverse: CCCCGTCTCTGTATTCAACC | ||||
| CD3 | 60 | 111 | Forward: AAGGGAGCCCCTTCAAGGTA | |
| Reverse: TCTTTGCAAACCATCCTTCC |
Tm, Temperature.