Table 3.
Proliferation of the patient 1's lymphocytes
| P1, CPM | C, CPM | Reference value, CPM | P1, SI | C, SI | Reference value, SI | |
|---|---|---|---|---|---|---|
| Media, control for PHA | 218 | 170 | ||||
| PHA, μg/mL | ||||||
| 5 | 242 | 49 431 | > 27 000 | 1 | 291 | |
| 2.5 | 269 | 32 403 | > 14 000 | 1 | 191 | |
| 1 | 139 | 12 142 | > 2000 | 1 | 71 | |
| Media, control for ConA | 309 | 170 | ||||
| ConA, μg/mL | ||||||
| 5 | 296 | 15 402 | > 4500 | 1 | 91 | |
| 1.7 | 336 | 2663 | > 1400 | 1 | 16 | |
| 0.6 | 259 | 410 | > 350 | 1 | 2 | |
| Media, control for PWM | 275 | 170 | ||||
| PWM, μg/mL | ||||||
| 25 | 362 | 7174 | > 3800 | 1 | 42 | |
| 8.3 | 322 | 13 795 | > 3500 | 1 | 81 | |
| 2.8 | 223 | 12 467 | > 3000 | 1 | 73 | |
| Media, control for Candida | 282 | 1054 | ||||
| Candida, 6.5 K PNU | 242 | 54 902 | 0.9 | 52.1 | > 5 | |
| Media, control for TET | 229 | 894 | ||||
| TET, LFU | ||||||
| 0.2 | 353 | 10 436 | 1.5 | 11.7 | > 5 | |
| 0.07 | 339 | 16 664 | 1.5 | 18.6 | > 5 | |
| 0.02 | 462 | 14 407 | 2 | 16.1 | > 5 |
PBMCs from P1 and a healthy control subject were incubated in the media alone or with ConA, PHA, PWM, Candida Ag, and TET Ag. [3H]Thymidine was added to cultures 6 hours before cell harvesting into filters. Filters were read by a liquid scintillation counter. SI is a ratio of [3H] thymidine counts for simulated cells divided by counts for unstimulated cells. Reference value represents counts or SI which are considered normal. Note that the Clinical Immunology Laboratory at National Jewish Health uses the CPM reference for PHA, ConA, and PWM, and the SI reference for Candida and TET.
P indicates patient; C, healthy control subject; CPM, counts per minute; SI, stimulation index; ConA, concanavalin A; PWM, pokeweed mitogen; PNU, protein nitrogen unit; TET, tetanus; and LFU, lytic forming unit.