Fig. 3.

Synthetic responses in basal and TNF-stimulated NHBE-ASM cocultures and in NHBE and ASM cells cultured alone. ELISAs were performed for inducible protein 10 (IP-10) secretion (A), IL-6 secretion (B), and fractalkine secretion (C) in ASM, NHBE, and NHBE-ASM cocultures from basolateral media following apical instillation of vehicle or TNF (10 ng/ml) for 24 h. Summarization of 3 separate experiments performed in triplicate. Data represent means ± SE from 3 separate experiments done in triplicate. Statistical evaluation was performed by ANOVA. P > 0.05, significant difference.