Fig. 6.

Ozone modulates PGE₂ levels in basal and TNF-stimulated NHBE-ASM cultures. NHBE-ASM cultures treated with TNF (10 ng/ml) or vehicle alone were exposed to ozone (black bars) or FA (gray bars) for 6 h. Immediately after this period, basolateral media was assayed by liquid chromatography-mass spectrometry (LC/MS) methodology as described in methods. For each prostanoid, data are expressed as fold changes over ozone-unexposed cultures. Data represent means ± SE from 3 separate experiments done in triplicate. Statistical evaluation was performed by ANOVA. P > 0.05, significant difference. 6K-PGF_1α, 6-keto-PGF_1α; TxB₂, thromboxane B₂.