Fig. 3.

Identification of metabolites generated from trans-ITZs by CYP3A4. A representative chromatogram of an incubation of cis-ITZ and trans-ITZ with CYP3A4. When incubated in the presence of CYP3A4 Supersomes, the new ITZ metabolites (M1 and M2) are formed from both cis-ITZ and trans-ITZ. The new metabolites were not found in the absence of CYP3A4 or NADPH control samples, as shown in the control traces. The MS data were collected by full scan using Synapt Q-TOF for high-accuracy MS. Ions monitored are m/z 100-1500 in low-energy mode, the cone voltage was 35 V, and the collision energy was 6 eV, as described under Materials and Methods.