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Determination of the dissociation rate for (2S,4S,2′S)-ITZ from CYP3A4 using SPR. The dissociation rate (koff) of (2S,4S,2′S)-ITZ was determined by one phase decay model, and the koff was found to be 0.0072 ± 0.0006 s−1. Only the dissociation phase of the SPR run is shown.
