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Rate of NAC-BDA-NAL formation catalyzed by human recombinant P450s. Furan (black bars, 20 μM; white bars, 400 μM) was incubated with human recombinant P450s (1 or 2 pmol P450/μl) in the presence of 8 mM NAC, 8 mM NAL, and an NADPH-regenerating system for 10 min at 37°C. Control = Supersomes expressing only NADPH reductase. Error bars represent S.E.
