Fig. 9.

Inhibition of glucagon synthesis by the 2,5-dideoxystreptamine derivative 166830 and the pyrrolidine bis-piperazine 1435-6 in α-TC6 cells. A, glucagon RIA. α-TC6 cultures were incubated with compounds 166830 and 1435-6 at a final concentration of 75 μM for 36 h. Cell extracts were then collected for total glucagon determination by RIA. Data represent the mean ± S.D. from quadruplicate wells from a representative experiment. *, significantly less (p < 0.0001) than the values obtained from control cultures incubated with the vehicle, Opti-MEM. B, cytotoxicity assay. In a parallel experiment, the WST-1 assay was used to determine the viability of α-TC6 cells after treatment with the inhibitors used above at the same final concentrations. The experiment was carried out using quadruplicate wells, and the data represent the mean ± S.D.