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. 2012 Mar;340(3):688–697. doi: 10.1124/jpet.111.188854

Fig. 2.

Fig. 2.

BMS-665351-mediated induction of CYP3A4 expression in human primary hepatocytes. Human primary hepatocyte cultures from donors were treated for 24 h with RIF (10 μM), CITCO (1 μM), or BMS-665351 (1 and 5 μM). A and B, total RNA was collected, reverse-transcribed, and subjected to real-time RT-PCR for detecting the CYP3A4 [donor HL-18 (A) and donor HL-19 (B)] expression levels as outlined under Materials and Methods. RT-PCR data are expressed as mean ± S.D. (n = 3). *, P < 0.05; **, P < 0.01. C and D, in separate experiments, HPHs from donors HL-21 (C) and HL-22 (D) were treated with the same batch of chemicals as indicated in A and B for 72 h. After harvesting, whole-cell homogenates (20 μg/each) were subjected to CYP3A4 immunoblotting analysis as described under Materials and Methods.