Fig. 1.

Binding of γ3-targeted nanocarriers to immobilized ICAM-1. FITC-labeled γ3 NCs (∼130 nm) were incubated for 15 min with control BSA, purified human ICAM-1 (hICAM-1), or mouse ICAM-1 (mICAM-1), which had been immobilized on nitrocellulose. The membranes were then washed and analyzed by fluorescence microscopy to quantify the number of nanocarriers bound per area. As a control, ICAM-1 binding of anti-ICAM NCs (clone R6.5) is shown (dashed line). Data are mean ± S.E.M. (n = 4). ***, p < 0.001, by Student's t test (compared with BSA).