Table 1.
Antibodies used for immunocytochemistry
| Primary antibody | Antibody type | Target | Dilution | Pretreatment | Source |
|---|---|---|---|---|---|
| NOX1 | Rabbit (pAB) | NADPH-Oxidase subunit | 1:200 | EDTA pH 9 | Sigma-Aldrich, SAB 4200097 |
| gp91phox | Mouse (mAB) | NADPH-Oxidase subunit | 1:100 | Citrate pH6 | Verhoeven et al., 1989 |
| p22phox | Rabbit (pAB) | NADPH-Oxidase subunit | 1:100 | Citrate pH6 | Santa Cruz, sc-20781 |
| NOXO1 | Rabbit (pAB) | NADPH-Oxidase subunit | 1:200 | Citrate pH6 | Sigma-Aldrich, SAB 2900367 |
| p47phox | Goat (pAB) | NADPH-Oxidase subunit | 1:100 | Citrate pH6 | Abcam, ab 74095 and Lifespan Biosci, LS-B2365 |
| GFAP | Mouse (mAB) | NADPH-Oxidase subunit | 1:200 | Citrate pH6 | Thermo Scientific, USA; MS1376 |
| CD68 | Mouse (mAB) | Macrophages, microglia | 1:100 | EDTA pH 9 | Dako, M0814 |
| IBA-1 | Rabbit (pAB) | Microglia | 1:3000 | EDTA pH9 | WAKO Chemicals, 019-19741 |
| LN3 | Mouse (mAB) | MHCII | 1:50 | Citrate pH6 | Dako |