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Expression of NADPH subunits in microglia and astrocytes (confocal microscopy double staining)
| p22phox | gp91phox | p47phox | NOX1 | NOXO1 | |
|---|---|---|---|---|---|
| Microglia (Iba1 or CD68) | + | + | + | + | + |
| Astrocytes (GFAP) | − | − | − | + | + |
Immunofluorescence was performed by double staining with markers for NADPH oxidase subunits and for microglia (Iba1 and CD68) or astrocytes (GFAP). Stained sections were analysed by confocal laser microscopy as shown in Fig. 2. GFAP = glial fibrillary acidic protein.
