Figure 4.

Analysis of proliferation and cell death in ERRN and WPERRN tumors. BrDU immunohistochemical staining was performed on end-stage tumors (> 2 cm³) (A) and mice with neoplasias at 220 days old (C). Bars represent the average number of positive cells per field of view (0.25 mm²). Pictures (E) and (F) correspond to ERRN (n = 7) and WPERRN (n = 7) representative examples, respectively. TUNEL staining was performed on end-stage tumors (B) and 220 day old mice (D). Bars represent the average number of positive cells per field of view (0.25 mm²). Pictures (G) and (H) correspond to ERRN (n = 7) and WPERRN (n = 7) representative examples, respectively. Scale bar = 50 μm.