Table 1.
Gene | Sequences | Annealing temp. (°C) |
Cycle no. |
---|---|---|---|
Gel-based PCR, rat | |||
ApoE | F: TGT TGG TCC CAT TGC TGA CAG GAT | 60 | 25 |
R: TGG TGT TTA CCT CGT TGC GGT ACT | |||
βAPP | F: CCC TGA CGC AGT CGA CAA GT | 61 | 25 |
R: TGT CAT AAC CTG GGA CCG GAT | |||
GAPDH | F: CAA CGG ATT TGG CCG TAT TG | 61 | 25 |
R: TGG GGG TAG GAA CAC GGA A | |||
ICE | F: ACA AAG AAG GTG GCG CAT TTC | 61 | 28 |
R: CCT TGT TTC TCT CCA CGG C | |||
IL-1α | F: GAG TCA ACT CAT TGG CGC TTG | 60 | 27 |
R: GGG CTG ATT GAA ACT TAG CCG | |||
IL-1β | F: TGA CTC GTG GGA TGA TGA CG | 61 | 27 |
R: CTG GAG ACT GCC CAT TCT CG | |||
TNF | F: GCA CAG AAA GCA TGA TCC GAG | 64 | 28 |
R: CCT GGT ATG AAG TGG CAA ATC G | |||
Gel-based PCR, human | |||
ApoE | F: TTC TGT GGG CTG CGT TGC TG | 60 | 25 |
R: TAC ACT GCC AGG CGC TTC TG | |||
GAPDH | F: AGG TCG GAG TCA ACG GAT TTG | 61 | 25 |
R: TGG CAG GTT TTT CTA GAC GGC | |||
Real-time PCR (rat) | |||
ApoE | F: CTG GTT CGA GCC GCT AGT G | 60 | N.A. |
R: CCT GTA TCT TCT CCA TTA GGT TTG C | |||
18S | F: TTC GAA CGT CTG CCC TAT CAA | 60 | N.A. |
R: ATG GTA GGC ACG GCG ACT A |