. 2012 Feb 24;7(2):e31777. doi: 10.1371/journal.pone.0031777

Table 4. Glycolytic and cysteine synthase enzyme activities.

	− SNP	+ SNP
ENZYME	Control enzyme activity	Remaining activity
	(mU/mg cellular protein)	(% of the control)
HK	23	100
PGI	323	100
GAPDH	635	99
PGAM	21–82^a	89
ENO	296–489^a	88
PPDK	141	94
NADH-ADH	617–785^a	26±7
NADPH-ADH	14–66^b	48±6
Malic enzyme	201–376^b	66±2
Cysteine synthase	980±110^b	145±13^*

Interval of activities in two (^a) and four (^b) independent extracts.

Two-tailed Student's t-test for non-paired samples, p<0.02 versus control amebas. One unit of enzyme activity is 1 µmol of substrate transformed in 1 min.

Trophozoites were incubated for 1 h in culture medium in the absence or presence of SNP. Clarified extracts were prepared under anaerobic conditions and enzymatic activities were then determined. Remaining activities were determined as the ratio of the results obtained after SNP treatment and for untreated parasites.