Figure 5. Determination of the fluorescence quantum yield of thioflavine T (ThT) bound to fibril.

(a) Optical density of ThT bound to amyloid fibril as superposition of optical densities of the dye bound in mode 1 (D_b1) and mode 2 (D_b2). (b) 3D dependence of D_b1q_b1+D_b2q_b2 from D_b1 and D_b2. Experimental data, best fit curve and the values of q_b1 and q_b2, determined multiple nonlinear regression are presented. (c) Reduced fluorescence intensities of ThT/W as superposition of the reduced fluorescence intensities of the dye bound to mode 1 (D_b1q_b1) and mode 2 (D_b2q_b2).