Table 1. Characteristics of thioflavin T bound to amyloid fibrils, acetylcholinesterase and free dye in water solution.
Object | λmax, nm | mode | εi, max×10−4, M−1 cm−1 | εi, 435×10−4, M−1 cm−1 | Kbi×10−5, M−1 | ni | qi |
Insulin fibrils | 450 | 1 | 8.7 | 5.7 | 200 | 0.01 | 0.83 |
2 | 3.5 | 2.6 | 1.2 | 0.08 | 0.30 | ||
Lysozyme fibrils1) | 449 | 1 | 5.1 | 3.7 | 75 | 0.11 | 0.442) |
2 | 6.7 | 5.8 | 0.56 | 0.24 | 5×10−42) | ||
Acetylcholinesterase | 420 | 1 | 2.4 | 1.9 | 0.082 | 1.1 | 0.036 |
Thioflavin T in water solution3) | 412 | - | 3.2 | 2.0 | - | - | 0.0001 |