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. 2012 Feb 24;7(2):e32492. doi: 10.1371/journal.pone.0032492

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Yu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescent staining of Caco-2 cells in 2D and 3D culture with anti-α6-integrin and ZO-1 antibodies. (B) Caco-2 cells in 3D culture were stained for differentiation markers indicating cell polarity and cyst formation (definition of lumen-cyst vesus cyst structures). (C) Left: western blotting showing knockdown of KLF4 in Caco-2 cells. Right: statistical analysis of percentage of lumen-cyst formation in Caco-2 cell 3D cultures, comparing between control and KLF4 siRNA-transfected cell cultures. (*, P<0.05) (D) Left: western blotting showing expression of KLF4 in 293T cells co-transfected with human KLF4 and KLF4 shRNA plasmids. Right: statistical analysis of percentage of lumen-cyst formation in Caco-2 cell 3D cultures, comparing between control and KLF4 shRNA-infected cell cultures. (*, P<0.05).