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. 2012 Feb 24;7(2):e32492. doi: 10.1371/journal.pone.0032492

Figure 4. KLF4 facilitates cell polarity and crypt-cyst formation in colon cancer cells.

Figure 4

(A) LS174 cells in 3D culture system were stained for differentiation markers indicating cell polarity and cyst formation. (B) Statistical analysis of percentage of cyst formation in LS174T cell 3D cultures, comparing between doxycycline (Dox)-induced and non-induced cells.(*, P<0.05) (C) Western blotting indicating expression of KLF4 in LS174T cell line with or without induction of doxycycline. (D) Semi-quantitative RT-PCR showing expression of genes related to KLF4-regulated cell polarity and related to Wnt signaling.