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Expression of osteoblast- and osteocyte-related marker genes. RT–PCR was used to analyze the expression of the indicated cell-marker genes in bone cells isolated in fraction (Fr) 5–7 from the freeze–thaw (FT) groups FT0 and FT1. GAPDH, glyceraldehydes-3-phosphate dehydrogenase; ALP alkaline phosphatase, PTHr parathyroid hormone receptor, COL1 alpha-1 type I collagen, OCN osteocalcin, SOST sclerostin, and DMP-1 dentin matrix protein 1
