Table 1.

Primer pairs used for PCR amplification

Target gene	Accession no.a	Primer pair sequences (sense/antisense)b	Base pairs	Cycles
OCN	X04141	5′-TGAGGACCCTCTCTCTGCTC-3′	127	30
		5′-ACCTTACTGCCCTCCTGCTT-3′
SOST	AF326741	5′-CAGCTCTCACTAGCCCCTTG-3′	228	30
		5′-CTCGGACACGTCTTTGGTGT-3′
DMP-1	NM_203493	5′-AGTTCGATGATGAGGGGATG-3′	299	30
		5′-AGTCTCGCTCCTGCTTTCCT-3′

OCN Osteocalcin, SOST sclerostin, DMP-1 dentin matrix protein-1

^aGenbank accession number of the sequences used in designing the primers

^bPrimer sequences were designed using BLUEPHIN software (Sigma Genosys, Japan)