Fig. 2.

Effects of LPS (positive control), T. vaginalis isolates (ATCC 30236 and TV-LACM2) and iNOS inhibitor (L-NMMA) on NO production by neutrophils. Neutrophils and parasites alone did not promote activation in NO production. LPS and T. vaginalis isolates were able to increase the NO release, while l-NMMA reversed the effect. Bars represent the mean ± S.D. of at least three independent experiments. Data were analyzed by ANOVA followed by Tukey's test (p < 0.05). Symbols represent difference when compared with: (asterisk) neutrophils alone (control); (sectionsign) T. vaginalis isolates; (number sign) neutrophils stimulated with T. vaginalis isolates