Fig. 3.

Treatment with ATP reduces the tumor spheres grown in neural stem cell (NSC) medium in different glioma cell lines. One thousand cells were grown in a 96-well plate for 7 days in the presence or absence of 100 μM of ATP in NSC medium. The number of spheres is shown as average ± SEM at day 7 in U87 (n = 4) (a), C6 (n = 2) (b), or U343 (n = 5) (c). Bars represent SEM. t test values: *p < 0.05. Right panel: representative images of tumor spheres of control and cultures treated for 7 days with ATP