Table 1.
Number of spheres and the amount of cells from U87, C6, and U343 cells at day 7
| Cell line | Cells plated | 750 cells | 1,000 cells | |||
|---|---|---|---|---|---|---|
| Medium | Treatment | Spheres | % cellsa | Spheres | % cellsa | |
| U87 | D+FCS | Control | 7.7 ± 1.5 (6) | 100 | 11.6 ± 1.4 (6) | 100 |
| U87 | D+FCS | ATP | 3.7 ± 0.9* (6) | 79 ± 9 (3) | 3.9 ± 0.8* (6) | 61 ± 14 (3) |
| U87 | D+FCS | ADP | 5.6 ± 1.2 (4) | 83 ± 10 (3) | 11.5 ± 1.2 (4) | 92 ± 20 (3) |
| U87 | D+FCS | ADO | 9.2 ± 0.4 (3) | 89 ± 19 (3) | 6.7 ± 1.0 (3) | 66 ± 7 (3) |
| U87 | D+FCS | UTP | 8.9 ± 1.1 (4) | 75 ± 2 (3) | 11.1 ± 1.1 (4) | 73 ± 5 (3) |
| U343 | NSC | Control | 39.3 ± 1.8 (5) | 100 | ||
| U343 | NSC | ATP | 36.4 ± 4.22 (5) | 91.9 ± 7.6 (5) | ||
| C6 | NSC | Control | 290.4 ± 53.3 (2) | 100 | ||
| C6 | NSC | ATP | 100.7 ± 3.8 (2) | 66.8 ± 1 (2) | ||
Values expressed as average ± SEM (n)
D + FSC DMEM + 5% FCS, NSC neural stem cell medium supplemented with EGF, FGF, LIF, and B27
*p < 0.05 test
aC6 and U343 cells were grown in NSC and cell amount was evaluated at day 7 using protein assay. U87 were grown in DMEM plus 5% FCS and cell amount was evaluated by MTT. Number of experiments are in parenthesis