Fig. 3.

The specific agonist of P2Y₂ and P2Y₄ receptor UTPγS induced Ca²⁺ signals in the hCMEC/D3 cells. Since the cells did not express P2Y₄ receptor (Fig. 1) the signal is related to P2Y₂ receptors. Additionally, MRS2578 or NF340 the specific inhibitors of P2Y₆ and P2Y₁₁ respectively did not affect the UDP or the BzATP induced Ca²⁺ signal in hCMEC/D3 cells