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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Neurobiol Dis. 2011 Dec 11;45(3):930–938. doi: 10.1016/j.nbd.2011.12.012

Figure 2. Ferritin in prion disease affected brains is denatured.

Figure 2

(A) Fractionation in PBS reveals significantly more ferritin in the insoluble fraction of MSc samples relative to NH controls. PrP from both NH and MSc samples partitions in the insoluble fraction as expected. Actin mainly partitions in the insoluble fraction under these conditions. (B) Quantification by densitometry after normalization with actin reveals insignificant difference between soluble and insoluble fractions of ferritin from NH samples, while a significant amount of ferritin from MSc samples partitions in the insoluble fraction. n=5 for NH and 4 for MSc. ***p<.001.