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. Author manuscript; available in PMC: 2012 Feb 26.
Published in final edited form as: Cell Immunol. 2007 Sep 25;247(2):95–102. doi: 10.1016/j.cellimm.2007.07.006

Figure 2. DN Cbl increases phosphorylation of signaling intermediates in CD4 T cells upon TCR/CD28 engagement.

Figure 2

CAR Tg Th1 T cells were transduced with EV or Ad-DN Cbl. On the next day, transduced cells were stimulated with anti-CD3/CD28 antibody-coated beads for 0, 5, 15, and 30 min. Cells were then lysed for Western blotting for phospho- and total-ERK, -p38MAPK, -Akt, and -Vav. Each membrane was first blotted with the indicated phospho-antibody, then stripped and re-blotted with the total antibody.