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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Cell Signal. 2011 Dec 29;24(4):914–921. doi: 10.1016/j.cellsig.2011.12.014

Fig. 2. AlF4−-activated Gαq or Gα11 induces GFP-PKD plasma membrane translocation.

Fig. 2

A, COS-7 cells were cotransfected with either GFP-PKD (upper and middle panels), or GFP-PKD-ΔCys2 (lower panel), together with either Gαq (upper and lower panels) or Gα11 (middle panel), as indicated. After 48 h, the cells were imaged before and after stimulation with 10 μM AlF4− for 45 min. B, ALF4−-activated wild-type Gαq or constitutively active Gαq-QL mutant potently induces plasma membrane translocation of a distinct fusion protein with RFP attached to the C-terminal position of PKD (PKD-RFP), but not of the RFP protein. COS-7 cells were transfected with either RFP (lower panel, left) or PKD-RFP either alone (upper panel), together with Gαq (middle panel) or constitutively active mutant Gαq-QL (lower panel), and either left unstimulated or stimulated with 10 μM AlF4− as indicated, prior to imaging.