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Kinesin accumulates proximal and distal to the ligation in P7 animals. Sciatic nerves were immunoblotted with (+Kif5C) and without (−Kif5C) an anti-kinesin antibody (Kif5c) in both ligated and unligated sciatic nerves. The proximal and distal nerve segments are identified, and the approximate location of the ligature site is indicated in ligated and unligated nerves
