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. 2011 Oct 31;589(Pt 24):5965–5986. doi: 10.1113/jphysiol.2011.219378

Figure 1. Labelling of murine corticotrophs in vitro using lentiviral transduction of a POMC-eYFP fluorescent reporter.

Figure 1

A, representative confocal sections of dispersed anterior pituitary cells in primary culture 4 days following transduction with pomc-eYFP (top panels) or CMV-eYFP (bottom panels). The eYFP signal (left panels), ACTH immunoreactivity (middle panels) and merged images are shown. Scale bar is 10 μm. B, quantification of percentage of cells immunoreactive for ACTH (ACTH+) that are also labelled with eYFP (eYFP+) using either the POMC- or CMV- promoter lentiviral constructs. C, quantification of percentage of cells labelled with eYFP (eYFP+) that are also immunoreactive for ACTH (ACTH+) and that are also using either the POMC- or CMV-promoter lentiviral constructs. D, ACTH release from control (Cont) and POMC-eYFP (POMC) transduced cultures under basal and stimulated (CRH/AVP, 0.1 and 2 nm respectively) expressed as a percentage of the basal release in control (non-transduced) cultures. All data are means ± SEM, n > 3 independent experiments.