Table 1.

PCR Primer Pairs Used for Detection of Three Target Genes and a Housekeeping Gene

Gene	Primer sequence (5′>3′)	Probe
Aggrecan	F: CCCAACCAGCCTGACAACTT	ACGCAGTCCTCTCCAGTGGCGAA
	R: CCTTCTCGTGCCAGATCATCA
Col Iα1	F: AGAAGAAGACATCCCACCAGTCA	AACGGCCTCAGGTACCATGACCGA
	R: AGATCACGTCATCGCACAACA
Col IIα1	F: CAGGTGAAGGTGGGAAACCA	ATCAGGGCGTTCCTGGTGAAGCTG
	R: ACCCACGAGGCCAGGA
GAPDH	F: TCGGAGTGAACGGATTTGG	CGCATCGGGCGCCTGGTC
	R: CCAGAGTTAAAAGCAGCCCT

Col Iα1, collagen type Iα1; Col IIα1, collagen type IIα1.